The matrix metalloproteinases （MMP） are a family of peptidase enzymes responsible for the degradation of extracellular matrix components，including collagen，gelatin，fibronectin，laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester，lipopolysaccharide （LPS） or staphylococcal enterotoxin B （SEB）. MMP catalysis requires both calcium and zinc. MMP-12 （also designated macrophage metalloelastase） is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.
應用類型
Western blotting （1-4 ug/ml）. However， the optimal concentrations should be determined individually.The antibody recognizes a ~50 kDa band from samples of human origin. Reactivity to other species has not been tested. Jurkat cell lysate （Cat.# 2401-100） can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 460 of human MMP-12
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的MMP-12蛋白 發生免疫反應.
保存建議
提供的MMP-12抗體 產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS緩沖液中.請將該MMP-12抗體 產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
