- 中文名稱
4-Hydroxynonenal (4-HNE) BioAssay ELISA Kit (Human)
- 英文名字
- 4-Hydroxynonenal (4-HNE) BioAssay ELISA Kit (Human)
- 供應商
- USBiological
- 產品貨號
- 353545
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The 4-Hydroxynonenal (4-HNE) BioAssay ELISA Kit (Human) is a quantitative competitive assay for the detection of 4-HNE in human serum, plasma, tissue homogenates and other biological fluids.Detection Range:0.625-40ng/mlSensitivity:<0.375ng/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with 4-HNE. 4-HNE in the standard or sample competes with a fixed amount of plate-coated 4-HNE for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin-HRP (SABC) is added to each well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of 4-HNE in the samples is determined by comparing the absorbance of the samples to the standard curve.Kit Components:*353545A: Microtiter Strips, 8x12 wells.*353545B: Standard, 2 vials353545C: Sample/Standard Dilution Buffer, 1x20ml353545D: Antibody-Biotin Concentrate, 1x160ul353545E: Antibody Dilution Buffer, 1x10ml353545F: Streptavidin-HRP Conjugate (SABC), 1x120ul353545G: SABC Dilution Buffer, 1x10ml353545H: TMB Substrate, 1x10ml353545J: Stop Solution, 1x10ml353545K: Wash Buffer (25X), 1x30mlStorage and Stability:Store unopened *353545A at 4oC; store at -20oC once opened. Store unopened *353545B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells.2. Add 50ul standard or sample to each well.3. Immediately add 50ul Antibody-Biotin working solution to each well and incubate for 45 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-20 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
- 產品特點
- 針對4-Hydroxynonenal (4-HNE) BioAssay ELISA Kit (Human)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到4-Hydroxynonenal (4-HNE) BioAssay ELISA Kit (Human)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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