Syntaxins were originally thought to be docking proteins，but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis，including VAMPs （vesicle-associated membrane proteins），NSF （N-ethylmaleimidesensitive factor），SNAP 25 （synaptosomal-associated protein of 25kDa），SNAPs （soluble NSF attachment proteins） and synaptotagmin. SNAPs mediate the membrane binding of NSF，which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.
應用類型
Western blotting （1-2 ug/ml）， immunoprecipitation （5-15 mg/ml） and Immunohistochemistry （10-20 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 85 kDa NSF of mouse， and rat origins. Reactivity to other species has not been tested
免疫原
Synthetic peptide corresponding to amino acids around 731 of rat NSF
來源宿主
兔
反應性
經檢測驗證，可與小鼠、大鼠種屬來源的NSF蛋白 發生免疫反應.
保存建議
提供的NSF抗體 產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02% 疊氮鈉的PBS緩沖液中.請將該NSF抗體 產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
