- 中文名稱
Choline O-acetyltransferase (Chat) BioAssay ELISA Kit (Rat)
- 英文名字
- Choline O-acetyltransferase (Chat) BioAssay ELISA Kit (Rat)
- 供應商
- USBiological
- 產品貨號
- 354759
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The Choline O-acetyltransferase (Chat) BioAssay ELISA Kit is a quantitative sandwich assay for the detection of Chat in rat serum, plasma, tissue homogenates and other biological fluids.Detection Range:0.312-20ng/mlSensitivity:<0.188ng/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to Chat. Standards and samples are added to the appropriate wells, then incubated. Aspirate and wash the plate. The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain Chat, the antibody (Biotin) and Streptavidin (HRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chat in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:354759A: Microtiter Strips, 1x96 wells (8x12 wells).*354759B: Standard, 2x1 vial354759C: Sample/Standard Dilution Buffer, 1x20ml354759D: Antibody (Biotin) (Concentrated), 1x120ul354759E: Antibody Dilution Buffer, 1x10ml354759F: Streptavidin (HRP) (SABC), 1x120ul354759G: SABC Dilution Buffer, 1x10ml354759H: TMB Substrate, 1x10ml354759J: Stop Solution, 1x10ml354759K: Wash Buffer, 25X, 1x30mlStorage and Stability:Store unopened *354759A at 4oC; store at -20oC once opened. Store unopened *354759B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards and samples to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times.3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37°C. 4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
- 產品特點
- The Choline O-acetyltransferase (Chat) BioAssay ELISA Kit is a quantitative sandwich assay for the detection of Chat in rat serum, plasma, tissue homogenates and other biological fluids.Detection Range:0.312-20ng/mlSensitivity:<0.188ng/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to Chat. Standards and samples are added to the appropriate wells, then incubated. Aspirate and wash the plate. The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain Chat, the antibody (Biotin) and Streptavidin (HRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chat in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:354759A: Microtiter Strips, 1x96 wells (8x12 wells).*354759B: Standard, 2x1 vial354759C: Sample/Standard Dilution Buffer, 1x20ml354759D: Antibody (Biotin) (Concentrated), 1x120ul354759E: Antibody Dilution Buffer, 1x10ml354759F: Streptavidin (HRP) (SABC), 1x120ul354759G: SABC Dilution Buffer, 1x10ml354759H: TMB Substrate, 1x10ml354759J: Stop Solution, 1x10ml354759K: Wash Buffer, 25X, 1x30mlStorage and Stability:Store unopened *354759A at 4oC; store at -20oC once opened. Store unopened *354759B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards and samples to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times.3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37°C. 4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
- 保存建議
- 建議收到Choline O-acetyltransferase (Chat) BioAssay ELISA Kit (Rat)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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