Tau，a microtubule-binding protein which serves to stabilize microtubules in growing axons，is found to be hyperphosphorylated in paired helical filaments （PHF），the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified，all of which are phosphorylated by GSK3. This presents the possibility that miscues in GSK3 signaling contribute to the onset of Alzheimer’s disease.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Other applications have not been determined. The antibody recognizes mainly the truncated Tau （~30 kDa） in sample from human， mouse and rat origins. Other isoforms of Tau at 35 kDa， 60 kDa and 80 kDa can also be recognized. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 395 of rat Tau
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、雞、牛種屬來源的Tau蛋白發生免疫反應.
保存建議
提供的Tau抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Tau抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
