The Toll-like receptor （TLR） family in mammalian comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Up-to-date，ten TLRs （TLR1-10） have been described. TLR2 is differentially expressed in human cells. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes，and CD19+ B-cells. CD3+ T-cells and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types is regulated by different immune response modifiers. For example，LPS，GM-CSF，IL-1，and IL-10 up regulate TLR2，whereas IL-4，IFN-gamma，and TNF down regulate TLR2 expression in monocytes.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes ~86 kDa TLR2 in Jurkat cell lysate. Reactivity to other species has not been tested. Blocking peptide （Cat.# 3552RBP-50） is available separately.
免疫原
Synthetic peptide surrounding amino acid 753 of mouse TLR2
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、猴種屬來源的TLR2蛋白發生免疫反應.
保存建議
提供的TLR2抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該TLR2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
