The Toll-like receptor （TLR） family of proteins are characterized by a highly conserved Toll homology （TH） domain，which is essential for Toll-induced signal transduction. TLR1，as well as the other TLR family members，are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride （LPS） stimulation，which results in the activation and translocation of NFkB and suggests that these receptors are involved in mediating inflammatory responses. TLR6 is highly homologous to TLR1，and like other members of TLR family，it induces NFkB signaling upon activation. Expression of TLR receptors is highest in peripheral blood leukocytes，macrophages，and monocytes.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Other applications have not been determined. The antibody recognizes ~97 kDa TLR5 in sample from human， mouse and rat origins. Reactivity to other species has not been tested. Blocking peptide （Cat.# 3555RBP-50） is available separately.
免疫原
Synthetic peptide surrounding amino acid 829 of human TLR5
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的TLR5蛋白發生免疫反應.
保存建議
提供的TLR5抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該TLR5抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
