TRADD (TNF-R1-associated death domain) is a 34 kDa protein that associates with the death domains of both FAS and TNF-R1 via their DDH regions. Overexpression of TRADD leads to NFκB activation and apoptosis in the absence of TNF.
應用類型
Western blotting (0.5-4 ug/ml). Based on researchers feedback, it also works well in immunoprecipitation (10-20 ug/ml) and Immunohistochemistry (15-25 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 34 kDa TRADD in samples from human, mouse, and rat origins. Reactivity to other species has not been tested. Mouse small intestine tissue lysate can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 300 of human TRADD
來源宿主
兔
反應性
經檢測驗證,可與人、小鼠、大鼠種屬來源的TRADD蛋白 發生免疫反應.
保存建議
提供的TRADD抗體 產品為100 ug 無色溶液,其濃度為0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該TRADD抗體 產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.