Galectins are a family of soluble B-galactoside-binding animal lectins that modulate cell-to-cell and cell-to-ECM interactions,as well as apoptosis. Galectin-3 encodes a 30-35 kDa protein containing a single carbohydrate binding domain,which binds galactose-containing glycoconjugates. Upregulated expression of Galectin-3 is involved in cancer progression and metastasis.
應用類型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes the 30 kDa human Galectin-3 in Western blot analysis with Jurkat cell lysate.
免疫原
Synthetic peptide surrounding amino acid 188 of human Galectin-3
來源宿主
兔
反應性
經檢測驗證,可與人種屬來源的Galectin-3蛋白發生免疫反應.
保存建議
提供的Galectin-3抗體產品為100 ug 無色溶液,其濃度為0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Galectin-3抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.