Due to the various splice variants，the trk proteins exist as variably glycosylated entities with the major forms having molecular weights of 140-145 kDa，110 kDa and the unglycosylated form of 80 kDa. trkB contains 33.3% carbohydrate by weight representing modification on 10 of 12 N-glycosylation sites. The primary ligand for trkB is BDNF which induces the phosphorylation of the protein and subsequent binding of PLC-gamma via SH2 domains. TrkB may function to modulate neuronal responses to the neurotrophins acting through trkB such as BDNF.
應用類型
Western blotting （0.5-4 ug/ml）， immunofluorescence （5-10 ug/ml）. However， the optimal concentrations should be determined individually. Trk protein exists as variably glycosylated entities with the major forms having molecular weights of 140 kDa， 110 kDa， and the unglycosylated form of 80 kDa.
免疫原
Synthetic peptide surrounding amino acid 810 of human TrkB
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、雞種屬來源的TrkB蛋白發生免疫反應.
保存建議
提供的TrkB抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該TrkB抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
