Aromatase is a key enzyme in steroidogenesis and plays an important role in sexual differentiation,fertility,and carcinogenesis. Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function.
應用類型
Western blotting (0.5-4 ug/ml), Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody should recognize aromatase in samples from human, mouse, rat, bovine, porcine, pig, sheep, rabbit, horse and chicken origins. A 55 kDa band can be detected, corresponding to the expected molecular weight of Aromatase. An additional 35 kDa band (believe to be the cleavage product of Aromatase) can also be detected in Jurkat cells.
免疫原
Synthetic peptide surrounding amino acid 385 of human Aromatase
來源宿主
兔
反應性
經檢測驗證,可與人、小鼠、大鼠、牛、雞、豬、兔、馬種屬來源的Aromatase蛋白發生免疫反應.
保存建議
提供的Aromatase抗體產品為100 ug 無色溶液,其濃度為0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Aromatase抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.