Interleukin 10 （IL-10），initially designated cytokine synthesis inhibitory factor （CSIF），was originally identified as a product of mouse T helper （Th） 2 clones that inhibited cytokine production by Th1 clones. The human ortholog of mouse IL-10 was subsequently cloned by cross-hybridization. Human and mouse IL-10 are 81% identical at the nucleotide and amino acid level. IL-10 is the prototypic member of the IL-10 cytokine family，including IL-10，IL-19，IL-20，IL-22 （IL-TIF），IL-24 and IL-26. A number of viruses，including Epstein-Barr virus （EBV） and human cytomegalovirus （HCMV），also encode viral members of the IL-10 family. Human IL-10 has cross-species actvity and is active on mouse cells. Mouse IL-10 is species-specific and does not act on human cells.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human IL-10 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human IL-10 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：7pg/mlRange：15.625-1000pg/mlSpecificity：Recognizes human IL-10.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h IL-10 monoclonal coating antibody is adsorbed onto microwells. IL-10 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h IL-10 antibody is added and binds to IL-10 captured by the first antibody. Following incubation unbound Biotinylated anti-h IL-10 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-10 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-10 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-10 standard dilutions and IL-10 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x12Standard： 2xVialBiotinylated antibody： 2xVialStreptavidin-HRP： 2xVialAssay Buffer （25ml/Vial）： 2xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x12mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 3xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
