HDAC4 is a member of the class II mammalian histone deacetylases，which consists of 1084 amino acid residues. Its C-terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4，unlike other deacetylases，shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC 4 protein in the cytoplasm. In the nucleus，HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects ~140 kDa human HDAC-4. Reactivity to other species has not been determined.
免疫原
Synthetic peptide surrounding amino acid 10 of human HDAC-4
來源宿主
兔
反應性
經檢測驗證，可與人、雞種屬來源的HDAC4蛋白發生免疫反應.
保存建議
提供的HDAC4抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該HDAC4抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
