Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.
應用類型
Western blotting （0.5-4 ug/ml）， Immunoprecipitation （20 ug/ml）， and Immunohistochemistry （20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects ~124 kDa HDAC5 with human and mouse samples， and in a lesser extent with rat samples. It does not cross-react with other HDAC proteins including HDAC1， 2， 3， 4， 6， 7， 8， 9，10， and 11.
免疫原
Synthetic peptide surrounding amino acid 575 of human HDAC-5
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的HDAC5蛋白發生免疫反應.
保存建議
提供的HDAC5抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該HDAC5抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
