CXCL8 was originally discovered and purified as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor （NCF），neutrophil activating protein （NAP），monocytederived neutrophil chemotactic factor （MDNCF），T lymphocyte chemotactic factor （TCF），granulocyte chemotactic protein （GCP） and leukocyte adhesion inhibitor （LAI）. Many cell types，including monocyte/macrophages，T cells，neutrophils，fibroblasts，endothelial cells，keratinocytes，hepatocytes，chondrocytes，and various tumor cell lines，can produce CXCL8 in response to a wide variety of proinflammatory stimuli such as exposure to IL-1，TNF，LPS，and viruses. CXCL8 is a member of the alpha （CXC） subfamily of chemokines，which also includes platelet factor-4，GRO，and IP10.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human IL-8 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human IL-8 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：2pg/mlRange：7.8-500pg/mlSpecificity：Recognizes human IL-8.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h IL-8 monoclonal coating antibody is adsorbed onto microwells. IL-8 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h IL-8 antibody is added and binds to IL-8 captured by the first antibody. Following incubation unbound Biotinylated anti-h IL-8 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-8 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-8 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-8 standard dilutions and IL-8 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x12Standard： 2xVialBiotinylated antibody： 2xVialStreptavidin-HRP： 2xVialAssay Buffer （25ml/Vial）： 2xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x12mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 3xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
