Human HDAC6 （1215 a.a. residues） possesses two separate putative catalytic domains，both of which are fully functional and contribute independently to the overall activity of HDAC6. A very potent NES is present at the amino-terminus of HDAC6，which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. HDAC6 may participate in coordinating expression of a group of genes involved in the remoldeling of chromatin during cell differentiation.
應用類型
Western blotting （0.5-4 ug/ml） and Immunohistochemistry （20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects ~134 kDa HDAC-6. Jurkat cell lysate can be used as a positive control. The antibody does not cross-react with other HDAC proteins including HDAC1， 2， 3， 4， 5， 7， and 8.
免疫原
Synthetic peptide mapping to the N-terminus of mouse HDAC-6
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的HDAC6蛋白發生免疫反應.
保存建議
提供的HDAC6抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該HDAC6抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
