Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus，it is also found in the cytoplasm，suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions，leading to the inhibition of gene transcription.
應用類型
Western blotting （0.5-4 ug/ml）， immunoprecipitation （20-40 ug/ml） and Immunohistochemistry （20-40 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects ~105 kDa HDAC7. Mouse small intestine tissue lysate can be use as a positive control. The antibody does not recognize other members of the HDAC family.
免疫原
Synthetic peptide surrounding amino acid 921 of murine HDAC7
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的HDAC7蛋白發生免疫反應.
保存建議
提供的HDAC7抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該HDAC7抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
