The matrix metalloproteinases （MMPs） consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix （ECM）. Additional MMP substrates include cytokines，chemokines，growth factors and binding proteins，cell/cell adhesion molecules，and other proteinases. With a few exceptions，MMPs share common structural motifs including a pro-peptide domain，a catalytic domain，a hinge region，and a hemopexin-like domain. Synthesized as pro-enzymes，most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription，pro-enzyme activation，or by their endogenous inhibitors，tissue inhibitors of metalloproteinases （TIMPs）. A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases （MT-MMP）.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human MMP-3 total BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human MMP-3 total concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：15pg/mlRange：62.5-4000pg/mlSpecificity：Recognizes human MMP-3 total.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h MMP-3 monoclonal coating antibody is adsorbed onto microwells. MMP-3 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h MMP-3 antibody is added and binds to MMP-3 captured by the first antibody. Following incubation unbound Biotinylated anti-h MMP-3 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h MMP-3 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of MMP-3 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MMP-3 standard dilutions and MMP-3 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x12Standard： 2xVialBiotinylated antibody： 2xVialStreptavidin-HRP： 2xVialAssay Buffer （25ml/Vial）： 2xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x12mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 3xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對MMP-3 total BioAssay ELISA Kit (Human) (Stromelysin-1，SL-1，Matrix metalloproteinase-3，MMP 3，Transin-1，MMP3，STMY1)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
保存建議
建議收到MMP-3 total BioAssay ELISA Kit （Human） （Stromelysin-1，SL-1，Matrix metalloproteinase-3，MMP 3，Transin-1，MMP3，STMY1）產品后將其置于4°C至-20°C保存。
