- 中文名稱
TNF-alpha BioAssay ELISA Kit (Human) (Tumor necrosis factor，Cachectin，TNF-alpha，Tumor necrosis factor ligand superfamily member 2，TNF-a，TNF，TNFA，TNFSF2，TNFalpha，TNF alpha，TNF a，TNFa)
- 英文名字
- TNF-alpha BioAssay ELISA Kit(Human)(Tumor necrosis factor，Cachectin，TNF-alpha，Tumor necrosis factor ligand superfamily member 2，TNF-a，TNF，TNFA，TNFSF2，TNFalpha，TNF alpha，TNF a，TNFa)
- 供應商
- USBiological
- 產品貨號
- 361329
- 產品報價
- ￥1/48Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The prototype ligand of the TNF superfamily，TNF-alpha/TNFSF1A，is a pleiotropic cytokine that plays a central role in inflammation and apoptosis. It is synthesized as a 26kD，type II transmembrane protein that is 233aa in length. It contains a 30aa cytoplasmic domain，a 26aa transmembrane segment，and a 177aa extracellular region. TNF-alpha is assembled intracellularly to form a transmembrane，non-covalently-linked homotrimeric protein. Human cells known to express TNF-alpha include B cells，colonic columnar epithelial cells，NK and CD3+CD56+ hepatic natural T cells，macrophages，monocytes and monocyte-derived dendritic cells，CD4+ and CD8+ T cells，mast cells，neutrophils，keratinocytes，plasma cells，and adipocytes.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human TNF-alpha BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human TNF-alpha concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：4pg/mlRange：15.625-1000pg/mlSpecificity：Recognizes human IFN-alpha. There was no detectable cross reactivity with any of the tested proteins following IL-1,2,3,4,5,6，8,10,12,VEGF,TGF,TNF and ICAM.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h TNF-alpha monoclonal coating antibody is adsorbed onto microwells. TNF-alpha present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h TNF-alpha antibody is added and binds to TNF-alpha captured by the first antibody. Following incubation unbound Biotinylated anti-h TNF-alpha antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h TNF-alpha antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of TNF-alpha present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF-alpha standard dilutions and TNF-alpha sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x6Standard： 1xVialBiotinylated antibody： 1xVialStreptavidin-HRP： 1xVialAssay Buffer （25ml/Vial）： 1xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x6mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 2xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對TNF-alpha BioAssay ELISA Kit (Human) (Tumor necrosis factor，Cachectin，TNF-alpha，Tumor necrosis factor ligand superfamily member 2，TNF-a，TNF，TNFA，TNFSF2，TNFalpha，TNF alpha，TNF a，TNFa)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到TNF-alpha BioAssay ELISA Kit （Human） （Tumor necrosis factor，Cachectin，TNF-alpha，Tumor necrosis factor ligand superfamily member 2，TNF-a，TNF，TNFA，TNFSF2，TNFalpha，TNF alpha，TNF a，TNFa）產品后將其置于4°C至-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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