- 中文名稱
VEGF BioAssay ELISA Kit (Human) (Vascular endothelial growth factor，VEGF，Vascular permeability factor，VPF)
- 英文名字
- VEGF BioAssay ELISA Kit(Human)(Vascular endothelial growth factor，VEGF，Vascular permeability factor，VPF)
- 供應商
- USBiological
- 產品貨號
- 361427
- 產品報價
- ￥1/48Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The gene for human VEGF is organized into 8 exons. As a result of alternative splicing，at least 4 transcripts encoding mature monomeric VEGF containing 121，165，189，and 206aa residues （VEGF121，VEGF165，VEGF189，and VEGF206）. VEGF is a highly conserved protein that has cross-species activity. Between human，rat or bovine VEGF，84-94% sequence identity has been observed. In vitro，VEGF is a potent endothelial cell mitogen. In cultured endothelial cells，VEGF can activate phospholipase C and induce rapid increases of free cytosolic Ca2+. VEGF has also been shown to be chemotactic for monocytes and osteoblasts. Based on its in vitro and in vivo properties，VEGF is expected to play important roles in inflammation and during normal and pathological angiogenesis，a process that is associated with wound healing，embryonic development，and growth and metastasis of solid tumors.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human VEGF BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human VEGF concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：4pg/mlRange：15.625-1000pg/mlSpecificity：Recognizes human VEGF. There was no detectable cross reactivity with any of the tested proteins following IL-1,2,3,4,5,6，8,10,12,TNF,TGF and IFN.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h VEGF monoclonal coating antibody is adsorbed onto microwells. VEGF present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h VEGF antibody is added and binds to VEGF captured by the first antibody. Following incubation unbound Biotinylated anti-h VEGF antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h VEGF antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of VEGF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven VEGF standard dilutions and VEGF sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x6Standard： 1xVialBiotinylated antibody： 1xVialStreptavidin-HRP： 1xVialAssay Buffer （25ml/Vial）： 1xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x6mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 2xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對VEGF BioAssay ELISA Kit (Human) (Vascular endothelial growth factor，VEGF，Vascular permeability factor，VPF)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到VEGF BioAssay ELISA Kit （Human） （Vascular endothelial growth factor，VEGF，Vascular permeability factor，VPF）產品后將其置于4°C至-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
-
- 注意
-
該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
-
