Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form，and is synthesized by many mesenchymal cells，for the extracellular matrix form. It is composed of two similar，but not identical protein chains，which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason，detection by immunoblot （western） may show considerable variability in the observed apparent molecular weights，which is predicated on the source of the fibronectin，and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used
應用類型
Western blotting （0.5 – 2.0 ug/ml）， Immunohistochemistry （20-40 ug/ml）. However， the optimal concentrations should be determined individually.
免疫原
Purified fibronectin from human plasma
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的Fibronectin蛋白發生免疫反應.
保存建議
提供的Fibronectin抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 0.1% BSA和0.02%硫柳汞的PBS緩沖液中.請將該Fibronectin抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
