Vimentin,a intermediate filament protein,is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family such as the cytokeratins,in neoplasm.
應用類型
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml) and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 448 of human Vimentin
來源宿主
兔
反應性
經檢測驗證,可與人、小鼠、大鼠、牛、雞種屬來源的Vimentin蛋白發生免疫反應.
保存建議
提供的Vimentin抗體產品為100 ug 無色溶液,其濃度為0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Vimentin抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.