Cytochrome c oxidase （COX） is a 13-subunit complex spanning the inner mitochondrial membrane and responsible for the terminal reduction of dioxygen to water in the electron transport chain. The three core catalytic units COX-1，-2，-3 are trans-membrane proteins encoded by the mitochondrial genome，whereas the remaining 10 subunits are nuclear encoded and expressed in a tissue-specific manner. The expression of nuclear and mitochondrial subunits of the mitochondrial respiratory chain is thought to be highly coordinated. Cox-4 is believed to regulate COX activity according to the extramitochondrial ATP/ADP ratio.
應用類型
Western blotting （0.5-4 ug/ml） and Immunohistochemistry （10-20 ug/ml）. However， the optimal concentrations should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 32 of human Cox IV
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Cox-4蛋白發生免疫反應.
保存建議
提供的Cox-4抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Cox-4抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
