MARCKS （Myristoylated alanine-rich protein kinase C substrate） is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility，cell adhesion，phagocytosis，membrane traffic and mitogenesis. PKC phosphorylates Ser152，156 and 163 of MARCKS，which regulates MARCKS’s calcium/calmodulin binding activity and filamentous （F）-actin cross-linking activity. In addition，phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm.
應用類型
Western blotting （1:200-1000）. However， the optimal conditions should be determined individually. Other applications have not been determined. The antibody detects 80 kDa MARCKS only when phosphorylated at Ser152/156.
免疫原
Synthetic peptide corresponding to amino acids surrounding Ser152/156 of rat MARCKS
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Phospho-MARCKS蛋白發生免疫反應.
保存建議
提供的Phospho-MARCKS抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Phospho-MARCKS抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
