Sample Type：Serum，plasma，tissue homogenates，cell lysates，cell culture supernates and other biological fluidsIntended Use：BioAssay ELISA Kit for Arachidonic Acid （AA） in serum，plasma，tissue homogenates，cell lysates，cell culture supernates and other biological fluidsDetection Range：2.47-200ug/mlSenstivity：<0.92ug/mlPrecision：Intra-Assay CV： <10%Inter-Assay CV： <12%Specificity：GeneralKit Components：*365733A： Microtiter Plate，96 wells，Pre-coated，ready to use.*365733B： Standard，2x1vial 365733C： Standard Diluent，1x20ml*365733D： Detection Reagent A，1x1vial *365733E： Detection Reagent B，1x120ul 365733F： Assay Diluent A，1x12ml365733G： Assay Diluent B，1x12ml365733H： TMB Substrate，1x9ml365733K： Stop Solution，1x6ml365733L： Wash Buffer，30X，1x20ml365733M： Reagent Diluent，1x300mlStorage and Stability：Store *365733A，*365733B，*365733D and *365733E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened，it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product，centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary：1. Prepare all reagents，samples and standards.2. Add 50ul standard or sample to each well，then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.5. Aspirate and wash 5 times.6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
