The Calcitonin Gene Related Peptide （CGRP） BioAssay ECL ELISA Kit is a sensitive chemiluminescent competitive enzyme immunoassay for the in vitro quantitative measurement of CGRP in human serum，plasma，tissue homogenates，cell lysates，cell culture superanatants and other biological fluids. Detection Range：7.8-2000pg/mlSensitivity：2.9pg/mlIntra-Assay CV： <10% Inter-Assay CV： <12%Kit Components：*365807A： Microtiter Plate，1x48 wells or 1x96 wells，Pre-coated with antibody; ready to use.*365807B： Standard，2x1vial 365807C： Standard Diluent，1x10ml or 1x20ml*365807D： Detection Reagent A （green），1x1vial*365807E： Detection Reagent B （red），1x60ul or 1x120ul 365807F： Assay Diluent A，1x6ml or 1x12ml365807G： Assay Diluent B，1x6ml or 1x12ml365807H： Substrate A，1x5ml or 1x10ml365807J： Substrate B，1x1ml or 1x2ml*365807K： Positive Control，1x1vial，350.8pg/ml （Lyophilized）365807L： Wash Buffer，30X，1x10ml or 1x20ml 365807M： Reagent Diluent，1x300ulPrecaution：The Stop Solution （365807K） suggested for use with this kit is an acid solution. Wear eye，hand，face，and clothing protection when using this material.Storage and Stability：Store *365807A，*365807B，*365807D，*365807E and *365807K at -20°C. After reconstitution of *365807K，store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened，it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product，centrifuge the original vials after thawing and prior to removing the cap.Notes on 365807K： Positive Control：Intended Use：The positive control is from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay，which indicates that the kit is optimized and working properly.Characteristics：The positive control vial contains CGRP in human serum （lyophilized powder）. Note： The human based serum used in this product are screened for HIV，Hepatitis A，B，C，HTLV and Syphilis.Expected Values：350.8pg/mlUsage： Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble，shake gently until fully dissolved. Mix gently before use. In the experiment，add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note： Upon receipt store Positive Control vial at -20°C. After reconstitution，store Positive Control vial at 4°C and use within 5 days.Assay Procedure Summary：1. Prepare all reagents，samples and standards.2. Add 50ul standard or sample to each well，then add 50ul Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 100ul Substrate Solution. Incubate 10 minutes at 37°C.8. Read RLU values immediately.
產品特點
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保存建議
建議收到Calcitonin Gene Related Peptide （CGRP） BioAssay ECL ELISA Kit （Human）產品后將其置于4°C至-20°C保存。
