Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density,intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/L.
應用類型
Western blotting (0.5-1 ug/ml), immunoprecipitation (5-10 ug/ml), Immunohistochemistry (5-10 ug/ml) and Elisa (0.5-1 ug/ml). However, the optimal conditions should be determined individually. The antibody reacts with human apoLipoprotein B. It shows negligible cross-reactivity with type A-I, A-II, C-I, C-II, C-III, E, and J apoLipoproteins (<1%).
免疫原
Apolipoprotein purified from human plasma
來源宿主
山羊
反應性
經檢測驗證,可與人種屬來源的ApoLipoprotein B蛋白發生免疫反應.
保存建議
提供的ApoLipoprotein B抗體產品為100 ug 無色溶液,其濃度為1.0 mg/ml,溶解于包含有50%甘油, 0.01%硫柳汞的PBS (pH 7.2)緩沖液中.請將該ApoLipoprotein B抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.