JIP1 （JNK-interacting protein-1） is a cytoplasmic protein that possesses an N-terminal JNK-binding domain and a C-terminal SRC homology 3 （SH3） domain. JIP1 selectively bind to JNK，but not to the ERK or p38 MAP kinases. In addition to JNK，JIP1 binds to specific proteins of JNK pathways，including MKK7，MLK3，and DLK，all at separate binding sites. JIP1 has been identified in brain and kidney tissues，whereas JIP2a，JIP2b，and JIP3 are specifically expressed in the brain. Overexpression of JIP1 causes cytoplamic retention of JNK，thereby preventing its nuclear accumulation and the subsequent activation of the JNK pathways，indicating that JIP1 acts as a cytoplasmic anchor for JNK.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 78 kDa JIP1 in samples from mouse， rat and human origins. In human sample， a ~110 kDa isoform can also be observed. Reactivity to other species has not been tested.
免疫原
Synthetic peptide from mouse JIP1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的JIP-1蛋白發生免疫反應.
保存建議
提供的JIP-1抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有0.5% BSA， 30%甘油和0.01%硫柳汞的PBS緩沖液中.請將該JIP-1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
