Noxa is a BH3-only member of the Bcl-2 family. Expression of Noxa gene involves direct activation of its promotor by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family，such as Bcl-xL and Mcl-1. Blocking the endogenous Noxa induction results in the suppression of apoptosis. These suggest Noxa may represent a candidate mediator of p53-induced apoptosis.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes mainly ~16 kDa band in human， mouse and rat samples. A ~34 kDa band is also detected to the lesser extent. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 100 of human Noxa
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Noxa蛋白發生免疫反應.
保存建議
提供的Noxa抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有0.5% BSA， 30%甘油和0.01%硫柳汞的PBS緩沖液中.請將該Noxa抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
