Mammalian forkhead members of the class O （FOXO） transcription factors，including FOXO1，FOXO3a，and FOXO4，are implicated in the regulation of a variety of cellular processes，including the cell cycle，apoptosis，DNA repair，stress resistance，and metabolism. FOXO proteins are negatively regulated by the phosphatidylinositol 3-kinase-Akt signaling pathway，which is activated by growth factors and cytokines. Recent studies indicate that the activities of FOXO proteins are also regulated by oxidative stress，which induces their phosphorylation，translocation to the nucleus，and acetylation-deacetylation. Similar to the tumor suppressor p53，FOXO is activated by stress and induces the expression of genes that contribute to cell-cycle arrest，suggesting that it also functions as a tumor suppressor.
應用類型
Western blotting （0.5-4 ug/ml）. Jurkat cell lysate （Cat.# 2401-100） and rat liver or rat kidney tissue lysate can be used as a positive control. A ~75 kDa band should be detected.
免疫原
Synthetic peptide corresponding to residues on the C-terminus of human FOXO3a
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的FOXO3a蛋白發生免疫反應.
保存建議
提供的FOXO3a抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有0.5% BSA， 30%甘油和0.01%硫柳汞的PBS緩沖液中.請將該FOXO3a抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
