HNF-4 （Hepatocyte nuclear factor 4），a member of the steroid/thyroid nuclear receptor superfamily，is a transcriptional factor which expresses in the liver，intestine，kidney，and pancreatic cells. It contains several functional domains: a ligand-independent activation domain （AF1），a zinc finger DNA binding domain，and a ligand-dependent activation domain （AF2）. HNF-4 binds to a specific DNA element as a homodimer and regulates the expression of many genes，involved in glucose，fatty acid，and cholesterol metabolisms.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. Mouse small intestine and rat kidney （Cat.# 2702-100） tissue lysates can be used as positive controls.
免疫原
Synthetic peptide surrounding amino acid 454 of rat HNF
來源宿主
兔
反應性
經檢測驗證，可與小鼠、大鼠種屬來源的HNF-4蛋白發生免疫反應.
保存建議
提供的HNF-4抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該HNF-4抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
