Lactate Dehydrogenase （LDH，EC 1.1.1.27） is a stable enzyme and is present in all cell types. Cell damage leads to release of LDH enzyme into the media and its activity is widely used as a marker for cytotoxicity. It reversibly converts lactate into pyruvate，with the concomitant interconversion of NADH and NAD+.Intended Use：Measurement of released LDH in culture mediumEvaluation of cytotoxicity effect of test compoundsSensitivity：~100 cellsSample Type：Adherent or Suspension CellsTest Principle：The LDH-Cytotoxicity Fluorometric BioAssay Kit provides a sensitive，quick，and easy way for detection of LDH released from damaged cells. In this assay，LDH converts lactate to pyruvate and NADH，which reduces a proprietary probe to an intensely fluorescent product （Ex/Em = 535/587 nm）. The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Kit Components：LDH Assay Buffer，1x50mlLDH Substrate Mix，lyophilizedPicoProbe，1x2mlCell Lysis Solution，1x5mlLDH Positive Control，1x1vialStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
