The neuronal calcium sensor (NCS) protein binds to calcium through EF-hand motifs,are expressed in neurons. They are implicated in the regulation of neurotransmitters released at the neuromuscular junctions. NCS-1 is widely distributed in the brain,spinal cord and dorsal root ganglia. It has been shown to regulate neurosecreation in a calcium-dependent manner,potentiates nitric oxide synthase activity. NCS-1 has been reserved to indicate the protein observed in humans and mammals.
應用類型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. Rat kidney tissue lysate can be used as a positive control. The antibody recognizes ~24 kDa NCS-1 from samples of human and rat origins. Reactivity to other species has not been determined.
免疫原
Synthetic peptide surrounding amino acid 180 of human NCS-1
來源宿主
兔
反應性
經檢測驗證,可與人、大鼠種屬來源的NCS-1蛋白發生免疫反應.
保存建議
提供的NCS-1抗體產品為100 ug 無色溶液,其濃度為0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該NCS-1抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.