Human MAP-1 （Modulator of Apoptosis Protein） is a novel Bax-associating protein. MAP-1 mediates caspase-dependent apoptosis in mammalian cells when overexpressed. MAP-1 homodimerizes and associates with the proapoptotic Bax and the prosurvival Bcl-2 and Bcl-XL of the Bcl-2 family.
應用類型
Western blotting （1-4 ug/ml）. However， the optimal concentrations should be determined individually. Jurkat cell lysate （Cat.# 2401-100） and mouse small intestine tissue lysate （Cat.# 2601-100） can be used as positive controls. The antibody recognizes 39 kDa MAP-1 from samples of human， mouse and rat origins. Reactivity to other species has not been determined. In human samples， an additional ~78 kDa dimer can be detected in Jurkat cell lysate. In mouse sample， an additional ~33 kDa cleavage fragment can also be detected in mouse small intestine tissue lysate.
免疫原
Synthetic peptide surrounding a.a. 342 of mouse MAP-1 （
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的MAP-1 蛋白發生免疫反應.
保存建議
提供的MAP-1 抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該MAP-1 抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
