XAF-1 binds to XIAP，an inhibitor of caspases-3，-7，-9 and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP’s ability to inhibit cell death. XAF-1is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast，XIAP levels have been shown to be high in a majority of cell lines.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. Jurkat cell lysate （Cat.# 2401-100） can be used as the positive control. The antibody recognizes 34 kDa XAF-1 from samples of human origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding a.a. 306 of human XAF-1
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的XAF-1蛋白發生免疫反應.
保存建議
提供的XAF-1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該XAF-1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
