The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines （such as TNF- and IL-1），UV light，inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1 （also known as MKK4），which in turn activates SAPK （also known as JNK）. JNK binds tightly to the N-terminal region of c-Jun and ATF-2，and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71.
應用類型
Western blotting （0.5-4 ug/ml），immunoprecipitation （10-20 ug/ml）， and Immunohistochemistry （10-20 ug/ml）. Reacts with human and to a lesser extent with mouse and rat. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 412 of human JNK-1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的JNK蛋白發生免疫反應.
保存建議
提供的JNK抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該JNK抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
