TUCAN （Tumor Up-regulated Card-containing Antagonist of Caspase-Nine） is a CARD （Caspase-Associated Recruitment Domains） containing protein that binds and suppresses activation of pro-Caspase-9 and found to be overexpressed in some types of cancers. TUCAN binds specifically to pro-caspase-9 and interferes with its interaction with Apaf-1，thereby suppressing apoptosis signaling in the mitochondrial/cytochrome c pathway.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects 47 kDa TUCAN in samples from human， mouse， and rat origins. Jurkat cell lysate， mouse small intestine and rat kidney tissue lysates can be used as positive controls.
免疫原
Synthetic peptide surrounding amino acid 420 of human TUCAN
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的TUCAN/CARD8蛋白發生免疫反應.
保存建議
提供的TUCAN/CARD8抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5 BSA和0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該TUCAN/CARD8抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
