DNA methylation can help regulate transcriptional silencing via repressive transcription complexes，which include methyl-CpG-binding domain protein and Histone deacetylases （HDACs） DNMT1. This core enzyme for mammalian DNA methylation can also establish a repressive transcription complex consisting of DNMT1，HDAC2 and DMAP1. DMAP1 can interact directly with the transcriptional corepressor TSG101. The DNMT1-DMAP1 exists throughout the S-phase； HDAC2 joind DNMT1 and DMAP1 only during Late S phase. This provides a regulated means to deacetylate heterochromatin following replication
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. Jurkat cell lysate （Cat.# 2401-100） and mouse small intestine tissue lysate （Cat.2601-100） can be used as positive controls. The antibody recognizes the DMAP1 of human， mouse and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 438 of mouse DMAP1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的DMAP1蛋白發生免疫反應.
保存建議
提供的DMAP1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 1% BSA和0.02%硫柳汞的PBS緩沖液中.請將該DMAP1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
