XLF （also called Cernunnos） interacts with the XRCC4-DNA ligase IV complex to promote DNA non-homologous end-joining. It directly interacts with the XRCC4-Ligase IV complex and siRNA-mediated downregulation of XLF in human cell lines leads to radio-sensitivity and impaired DNA non-homologous end-joining. XLF is homologous to the yeast non-homologous end-joining factor Nej1.
應用類型
Western blotting （1:500 – 1:2500） and Immunoprecipitation. However， the optimal concentrations should be determined individually. The antibody recognizes the XLF of human origin. Reactivity to other species has not been tested.
免疫原
Synthetic peptide of human XLF
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的XLF蛋白發生免疫反應.
保存建議
提供的XLF抗體產品為100 ul 無色溶液，其濃度為N/A，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該XLF抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
