PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3.Intended Use：Suitable for studying enzyme kinetics and HTS applications.Test Principle：The PRMT5 Chemiluminescent BioAssay Kit is designed to measure PRMT5 activity for screening and profiling applications. The PRMT5 Chemiluminescent BioAssay Kit comes in a convenient format，with a 384-well plate precoated with histone H4 peptide substrate，the antibody against methylated arginine3 residue of Histone H4，the secondary HRP-labeled antibody，S-adenosylmethionine，methyltransferase assay buffer，and purified PRMT5 enzyme for 384 enzyme reactions. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit，only three simple steps on a microtiter plate are required for methyltransferase detection. First，S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next，primary antibody is added. Finally，the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Contraindications： DMSO >1%，strong acids or bases，ionic detergents，high saltKit Components：PRMT5/MEP50 human enzyme，1x40ug100uM S-adenosylmethionine，1x250ulPrimary antibody 4-3，1x100ulSecondary HRP-labeled antibody 2，2x10ul4x PRMT5 assay buffer （add DTT before experiment），2x3mlBlocking buffer，2x50mlHRP chemiluminescent substrate A （transparent bottle），2x6mlHRP chemiluminescent substrate B （brown bottle），2x6ml384-well plate precoated with histone substrate，1x plateStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
