Intended Use：Suitable for studying enzyme kinetics and HTS applications.Test Principle：The PRMT9 Homogeneous BioAssay Kit is designed to measure PRMT9 activity for screening and profiling applications. PRMT9 is a histone methyltransferase that exhibits methylation activity toward an arginine residue of SAP145. The PRMT9 Homogeneous BioAssay Kit comes in a convenient AlphaLISA format，with GST-tagged PRMT9 substrate，primary antibody，methylation assay buffer，and purified PRMT9 for 384 enzyme reactions. The key to the PRMT9 Homogeneous BioAssay Kit is a highly specific antibody that recognizes methylated substrate. With this kit，only three simple steps on a microtiter plate are required for methyltransferase detection. First，a sample containing PRMT9 enzyme is incubated with the substrate. Next，acceptor beads and primary antibody are added，then donor beads，followed by reading the Alpha-counts.Contraindications：Green and blue dyes that absorb light in the AlphaScreen signal emission range （520-620nm），such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide （NaN3） or metal ions （Fe2+，Fe3+，Cu2+，Zn2+ and Ni2+）. The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM，which lacks these components，does not affect AlphaScreen assays.Kit Components：PRMT9，1x40ug250uM S-adenosylmethionine，1x400ulPrimary antibody 28，1x20ulPRMT9 substrate，1x200ul4x PRMT9 assay buffer，3x1ml4x Detection buffer，1x2mlProtective film （to seal the wells）Storage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
