Xenopus Dickkopf （Dkk）-1 was initially discovered as a Wnt antagonist that plays an important role in head formation. By far，four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of Dkk1 and Dkk2 inhibited Wnt-3a-activated signaling，whereas the first Cys-rich domains had no effects. In addition，the second Cys-rich domain of Dkk-2，but not that of Dkk-1，was able to activate the canonical pathway in the presence of LRP6，and this LRP-dependent signaling does not require Dvl.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes 57 kDa Dkk2 in samples from human， mouse and rat origins. A ~55 kDa and ~28 kDa Dkk2 fragments can also be detected in rat kidney tissue lysate. Reactivity to other species has not been determined.
免疫原
Synthetic peptide surrounding amino acid 241 of mouse Dkk2
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的DKK2蛋白發生免疫反應.
保存建議
提供的DKK2抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該DKK2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
