Calnexin (CNX) is a 90kDa integral protein of the endoplasmic reticulum (ER). Calnexin belongs among chaperones,which are characterized by their main function of assisting protein folding and quality control,ensuring that only properly folded and assembled proteins proceed further along the secretary pathway. The function of Calnexin is to retain unfolded or unassembled N-linked glycoproteins in the endoplasmic reticulum.
應用類型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~90-100 kDa band, corresponding to glycosylated Calnexin in samples from human, mouse, rat, and dog origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 580 of human Calnexin
來源宿主
兔
反應性
經檢測驗證,可與人、小鼠、大鼠、狗種屬來源的Calnexin蛋白發生免疫反應.
保存建議
提供的Calnexin抗體產品為100 ug 無色溶液,其濃度為0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Calnexin抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.