Diacylglycerol acyltransferase is also known as DGAT1 is a microsomal enzyme. It is involved in the synthesis of fatty acids into triglycerides. DGAT utilizes diacylglycerol (DAG) and fatty acyl CoA as substrates to synthesize triacylglycerol. DGAT plays an important role in the metabolism of cellular diacylglycerol and is essential in the physiologic processes of higher eukaryotes that includes triacylglycerol metabolism such as intestinal fat absorption,lipoprotein assembly,adipose tissue formation,and lactation.
應用類型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~60 kDa DGAT-1 of human, mouse, and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to residues surrounding amino acids 308 of mouse DGAT-1
來源宿主
兔
反應性
經檢測驗證,可與人、小鼠、大鼠種屬來源的DGAT1蛋白發生免疫反應.
保存建議
提供的DGAT1抗體產品為100 ug 無色溶液,其濃度為0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)緩沖液中.請將該DGAT1抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.