Ficolin-1 also known as M-Ficolin is a pattern recognition molecule of the lectin complement activation pathway. It is expressed mostly in monocytes，nuetrophils and in type II alveolar epithelial cells. Ficolin-1 is detected on the surface of circulating monocytes but is not present in the plasma. Ficolin-1 has a 28 aa signal sequence，an N-terminal collagen domain and a C-terminal fibrinogen-like （FBG） domain that contain a calcium binding site and a potential N-glycosylation site.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes ~33 kDa Ficolin-1 and a 37 kDa Ficolin-1 precursor in samples from human， mouse and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 320 of rat Ficolin-1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Ficolin-1蛋白發生免疫反應.
保存建議
提供的Ficolin-1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Ficolin-1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
