Insulin-induced gene （InSig） localizes in the endoplasmic recticulum（ER） and is highly expressed in the liver and fibroblast cell lines. InSig1 and InSig2 play important roles in the regulation of cholesterol biosynthesis. Sterol induces InSig1 binding to the sterol-sensing domain of SREBP cleavage-activating protein （SCAP）. Both InSig1 and Insig2 prevent the export of SCAP from the ER and thus，inhibit cholesterol synthesis by preventing the proteolytic cleavage of SREBPs by the Golgi enzymes.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes ~30 kDa InSig-1 and its precursor （~50 kDa） in samples from human， mouse and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 248 of rat Insig1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、牛、倉鼠種屬來源的Insig1蛋白發生免疫反應.
保存建議
提供的Insig1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Insig1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
