The Firefly luciferase is a genetically modified variant derived from the Luciferase of Diaphanes pectinealis endemic to Yunnan province,China. It has been modified to provide enhanced stability compared to the normal phenotype of Photinus pyralis as well as a broader and more physiologically relevant effective pH range. Luciferase has been used as a sensitive reporter for studying gene regulation and function. Firefly luciferase catalyzes the oxidative carboxylation of luciferin,a reaction with the highest efficiency of any known bioluminescence reaction.
應用類型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes Chinese firefly luciferase. Cross-reactivity to other types of Luciferases have not been determined. Recombinant Chinese firefly luciferase (Cat.# 7901-5) can be used as a positive control.
免疫原
Recombinant Chinese Firefly luciferase
來源宿主
兔
反應性
經檢測驗證,可與Luciferase種屬來源的Luciferase蛋白發生免疫反應.
保存建議
提供的Luciferase抗體產品為200 ug 無色溶液,其濃度為0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Luciferase抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.