SAPK/Erk kinase （SEK1），also known as MKK4 or Jun kinase kinase （JNKK），activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines. Activation of SEK1 occurs through phosphorylation of serine and threonine residues at positions 257 and 261，respectively，by MEKK. Like MEK，SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop. Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses the stress-activated signal transduction.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes unphosphorylated and phosphorylated SEK1 from samples of human， mouse， and dog origins.
免疫原
Synthetic peptide surrounding amino acid 347 of mouse SEK1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、狗種屬來源的SEK1/MKK4/JKK1蛋白發生免疫反應.
保存建議
提供的SEK1/MKK4/JKK1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該SEK1/MKK4/JKK1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
