Autophagy，the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. An ubiquitin like conjugation system is involve in the formation of autophagosome where by Atg12 is covanletly bound to Apg5/ATG5. The conjugation is mediated by the ubiquitin-E1-like enzyme Atg7 and thE2-like enzyme Atg10.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes ~37 kDa Apg5/Atg5 from samples of human， mouse and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 226 of rat Apg5
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、豬、牛種屬來源的Apg5/Atg5蛋白發生免疫反應.
保存建議
提供的Apg5/Atg5抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Apg5/Atg5抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
