Autophagy，the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR （Target of rapamycin） through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme； this function is conserved in the mammalian homolog （4）. In mammalian cells，APG7 is essential for autophagy conjugation systems，autophagosome formation，starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Other applications have not been determined.
免疫原
Synthetic peptide near the N-terminus of rat APG7
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的APG7/ATG7蛋白發生免疫反應.
保存建議
提供的APG7/ATG7抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5 mg/ml BSA和0.01%硫柳汞的PBS緩沖液中.請將該APG7/ATG7抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
